@Article{ecn.2016.0377,
AUTHOR = {Magdalena Paplinska-Goryca, Patrycja Nejman-Gryz, Małgorzata Proboszcz, Rafał Krenke},
TITLE = {The effect of 1,25-dihydroxyvitamin D3 on TSLP, IL-33 and IL-25 expression in respiratory epithelium},
JOURNAL = {European Cytokine Network},
VOLUME = {27},
YEAR = {2016},
NUMBER = {2},
PAGES = {54--62},
URL = {http://www.techscience.com/ECN/v27n2/65541},
ISSN = {1952-4005},
ABSTRACT = {Background: Airway epithelium is an active and important component of the immunological response
in the pathophysiology of obstructive lung diseases. Recent studies suggest an important role for vitamin D3 in asthma
severity and treatment response. Objective: Our study evaluated the influence of an active form of vitamin D3 on
the expression of selected mediators of allergic inflammation in the respiratory epithelium. Material and Methods:
Primary nasal and bronchial epithelial cells were exposed to1,25D3 for 1 hour and were then stimulated or not with
IL-4, TNF-α, LPS, and poly I:C. After 24 hours TSLP, IL-33, and IL-25 protein levels were measured in culture
supernatants using ELISA and mRNA levels in cells by real time PCR.Results: 1,25D3 increased TSLP concentration
in unstimulated nasal epithelial cells, but did not influence IL-33 and IL-25 expression. In IL-4-stimulated epithelial
cell cultures 1,25D3 mostly inhibited TSLP and IL-33 expression. In LPS-treated cultures 1,25D3 decreased IL-33
expression. Simultaneously 1,25D3 augmented IL-25 production in the same model of stimulation. Conclusion: Our
study revealed the dual nature of vitamin D3 manifested in both pro- and anti-inflammatory properties observed
in airway epithelial cells.},
DOI = {10.1684/ecn.2016.0377}
}