@Article{ecn.2016.0385,
AUTHOR = {Vyacheslav A. Shmarov, Vladimir V. Malashchenko, Maksim E. Meniailo, Natalia D. Gazatova, Natalia M. Todosenko, Olga B. Melashchenko, Andrei G. Goncharov, Victor I. Seledtsov},
TITLE = {Direct effects of interleukin-7 on the function of human T cells in vitro},
JOURNAL = {European Cytokine Network},
VOLUME = {27},
YEAR = {2016},
NUMBER = {4},
PAGES = {102--107},
URL = {http://www.techscience.com/ECN/v27n4/65531},
ISSN = {1952-4005},
ABSTRACT = {CD3+ T lymphocytes were isolated by positive magnetic separation from the peripheral blood of
healthy donors. In the absence of any additional activating stimuli, interleukin-7 (IL-7) was shown to augment
the levels of T cells expressing CD25 activation marker both in CD4-positive and in CD4-negative effector memory
(CD45RA-CD197-)
T cell subsets, as well as in terminally differentiated (CD45RA+CD197-) T cells, without
significantly affecting the activation status of naive (CD45RA+CD197+) and central memory (CD45RA-CD197+)
T
cells. In addition, IL-7 noticeably enhanced the production of IL-2, interferon-γ (IFN-γ), and IL-10, but not IL-4,
in T cells. The direct effects of IL-7 on T cell activation induced in vitro by MACSiBeadTM particles coated with
CD2, CD3, and CD28 antibodies (Abs) were also investigated. Upon cell activation, IL-7 significantly augmented
the levels of CD25+ T cells in naive (CD45RA+CD197+), central memory (CD45RA-CD197+),
and effector memory
(CD45RA-CD197-)
T-cell compartments. In addition, IL-7 facilitated activation of CD4- (but not CD4+) terminally
differentiated effector (CD45RA+CD197-) T cells. Finally, IL-7 was found to upregulate the production of IL-2,
IFN-γ, IL-4, and IL-10 by activated T cells. In conclusion, we speculate that IL-7 is capable of enhancing functional
T cell activity without causing significant functional inbalance between various T cell subsets.},
DOI = {10.1684/ecn.2016.0385}
}