TY - EJOU
AU - Shmarov, Vyacheslav A.
AU - Malashchenko, Vladimir V.
AU - Meniailo, Maksim E.
AU - Gazatova, Natalia D.
AU - Todosenko, Natalia M.
AU - Melashchenko, Olga B.
AU - Goncharov, Andrei G.
AU - Seledtsov, Victor I.
TI - Direct effects of interleukin-7 on the function of human T cells in vitro
T2 - European Cytokine Network
PY - 2016
VL - 27
IS - 4
SN - 1952-4005
AB - CD3+ T lymphocytes were isolated by positive magnetic separation from the peripheral blood of
healthy donors. In the absence of any additional activating stimuli, interleukin-7 (IL-7) was shown to augment
the levels of T cells expressing CD25 activation marker both in CD4-positive and in CD4-negative effector memory
(CD45RA-CD197-)
T cell subsets, as well as in terminally differentiated (CD45RA+CD197-) T cells, without
significantly affecting the activation status of naive (CD45RA+CD197+) and central memory (CD45RA-CD197+)
T
cells. In addition, IL-7 noticeably enhanced the production of IL-2, interferon-γ (IFN-γ), and IL-10, but not IL-4,
in T cells. The direct effects of IL-7 on T cell activation induced in vitro by MACSiBeadTM particles coated with
CD2, CD3, and CD28 antibodies (Abs) were also investigated. Upon cell activation, IL-7 significantly augmented
the levels of CD25+ T cells in naive (CD45RA+CD197+), central memory (CD45RA-CD197+),
and effector memory
(CD45RA-CD197-)
T-cell compartments. In addition, IL-7 facilitated activation of CD4- (but not CD4+) terminally
differentiated effector (CD45RA+CD197-) T cells. Finally, IL-7 was found to upregulate the production of IL-2,
IFN-γ, IL-4, and IL-10 by activated T cells. In conclusion, we speculate that IL-7 is capable of enhancing functional
T cell activity without causing significant functional inbalance between various T cell subsets.
KW - interleukin-7
KW - T cell
KW - homeostatic activity
KW - T-cell activation
KW - CD25
KW - cytokine production
DO - 10.1684/ecn.2016.0385