@Article{ecn.2016.0380,
AUTHOR = {Qi Wang, Hui Wang, Qiang Ju, Zhen Ding, Xing Ge, Qiao-Mei Shi, Ji-Long Zhou, Xiao-Long Zhou, Jin-Peng Zhang, Mei-Rong Zhang, Hong-Min Yu, Li-Chun Xu},
TITLE = {The co-regulators SRC-1 and SMRT are involved in interleukin-6-induced androgen receptor activation},
JOURNAL = {European Cytokine Network},
VOLUME = {27},
YEAR = {2016},
NUMBER = {4},
PAGES = {108--113},
URL = {http://www.techscience.com/ECN/v27n4/65532},
ISSN = {1952-4005},
ABSTRACT = {Background: The androgen receptor (AR) can be stimulated by interleukin-6 (IL-6) in the absence
of androgens to induce prostate cancer progression. The purpose of this study was to investigate whether the
co-activator steroid receptor coactivator-1 (SRC-1) and co-repressor silencing mediator for retinoid and thyroid
hormone receptors (SMRT) are involved in IL-6-induced AR activation. Methods: The effects of IL-6 on LNCaP
cell proliferation were monitored using real-time cell analysis (RTCA) iCELLigence system. The impacts of IL-6 on
the association of the AR with SRC-1 and SMRT were investigated using the mammalian two-hybrid assay. Results:
IL-6 increased the proliferation of LNCaP cells with maximal induction at 50 ng/mL. The AR-SRC-1interaction was
enhanced by IL-6, with maximal induction at the concentration of 50 ng/mL (P<0.05). IL-6 decreased the AR-SMRT
interaction and a marked reduction was detected at 50 ng/mL (P<0.05). Conclusions: IL-6 enhances LNCaP cells
proliferation, which suggests that IL-6 might cause AR-positive prostate cancer growth through activation of the AR.
The mechanism of IL-6-induced AR activation is mediated through enhancing AR-SRC-1 interaction and inhibiting
AR-SMRT interaction. We have shown a significant role for SRC-1 and SMRT in modulating IL-6-induced AR
transactivation.},
DOI = {10.1684/ecn.2016.0380}
}