TY  - EJOU
AU  - Wang, Qi 
AU  - Wang, Hui 
AU  - Ju, Qiang 
AU  - Ding, Zhen 
AU  - Ge, Xing 
AU  - Shi, Qiao-Mei 
AU  - Zhou, Ji-Long 
AU  - Zhou, Xiao-Long 
AU  - Zhang, Jin-Peng 
AU  - Zhang, Mei-Rong 
AU  - Yu, Hong-Min 
AU  - Xu, Li-Chun 

TI  - The co-regulators SRC-1 and SMRT are involved in interleukin-6-induced androgen receptor activation
T2  - European Cytokine Network

PY  - 2016
VL  - 27
IS  - 4
SN  - 1952-4005

AB  - Background: The androgen receptor (AR) can be stimulated by interleukin-6 (IL-6) in the absence
of androgens to induce prostate cancer progression. The purpose of this study was to investigate whether the
co-activator steroid receptor coactivator-1 (SRC-1) and co-repressor silencing mediator for retinoid and thyroid
hormone receptors (SMRT) are involved in IL-6-induced AR activation. Methods: The effects of IL-6 on LNCaP
cell proliferation were monitored using real-time cell analysis (RTCA) iCELLigence system. The impacts of IL-6 on
the association of the AR with SRC-1 and SMRT were investigated using the mammalian two-hybrid assay. Results:
IL-6 increased the proliferation of LNCaP cells with maximal induction at 50 ng/mL. The AR-SRC-1interaction was
enhanced by IL-6, with maximal induction at the concentration of 50 ng/mL (P<0.05). IL-6 decreased the AR-SMRT
interaction and a marked reduction was detected at 50 ng/mL (P<0.05). Conclusions: IL-6 enhances LNCaP cells
proliferation, which suggests that IL-6 might cause AR-positive prostate cancer growth through activation of the AR.
The mechanism of IL-6-induced AR activation is mediated through enhancing AR-SRC-1 interaction and inhibiting
AR-SMRT interaction. We have shown a significant role for SRC-1 and SMRT in modulating IL-6-induced AR
transactivation.
KW  - steroid receptor coactivator-1
KW  -  silencing mediator for retinoid and thyroid hormone receptors
KW  -  androgen receptor
KW  - interleukin-6

DO  - 10.1684/ecn.2016.0380