@Article{ecn.2015.0368,
AUTHOR = {Sifei Yu, Xi Luo, Binyan Yang, Li Xiao, Xingmei Wu, Huabin Li, Changyou Wu},
TITLE = {Poly-functional T helper cells in human tonsillar mononuclear cells},
JOURNAL = {European Cytokine Network},
VOLUME = {30},
YEAR = {2019},
NUMBER = {4},
PAGES = {114--122},
URL = {http://www.techscience.com/ECN/v30n4/65381},
ISSN = {1952-4005},
ABSTRACT = {Tonsils are important lymphoid organs in which B cells and T cells complete their maturation and
identify cells that are infected by pathogens. However, the functions of T cells in human tonsils remain unclear,
especially the characteristics of polyfunctional CD4<sup>+</sup> T helper cells. In this study, we used multi-color flow cytometry
to analyze the expression or co-expression of effector cytokines in CD4<sup>+</sup> T cells from tonsillar tissues. We have
demonstrated that tonsillar CD4<sup>+</sup> T cell can express various Th effector cytokines after short-term polyclonal
stimulation, and that cytokine-producing CD4<sup>+</sup> T cells were CD45RO+ T cells. In addition, we analyzed the co-expression
of two or more kinds of cytokines at the level of a single cell. The results showed that tonsillar CD4<sup>+</sup>
T cells exhibited polyfunctionality by co-expressing two to five kinds of cytokines in the same time. These data
furnished a basic theory for further understanding the differentiation of polyfunctional Th cells in human tonsils
and their functions in resisting invasive microorganisms.},
DOI = {10.1684/ecn.2015.0368}
}