TY  - EJOU
AU  - (Wojciechowska), Joanna Krajewska 
AU  - Kos·cielska-Kasprzak, Katarzyna 
AU  - Krajewski, Wojciech 
AU  - Morawski, Krzysztof 

TI  - Serum levels of interleukin-32 and interleukin-6 in granulomatosis with polyangiitis and microscopic polyangiitis: association with clinical and biochemical findings
T2  - European Cytokine Network

PY  - 2019
VL  - 30
IS  - 4
SN  - 1952-4005

AB  - Background: Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis is an autoimmune
disorder of unknown etiology with dysregulated cytokines levels. Objectives: The main aim of this study was to assess the
clinical correlation between antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis, granulomatosis with
polyangiitis (GPA) serum levels of the microscopic polyangiitis (MPA), serum levels of the proinflammatory cytokines,
interleukin (IL)-32 and interleukin-6. Methods: Study included 71 patients, 47 with GPA and 24 with MPA. Serum IL-32
and IL-6 concentrations were analyzed in all patients, and compared with levels observed in 10 controls. IL-32 and IL-6
were evaluated using DuoSet and Quantikine HS ELISA, respectively. IL-32 and IL-6 concentrations were correlated
with disease-related clinical and laboratory findings. Results: IL-32 and IL-6 levels were significantly higher in GPA and
MPA than in controls, especially IL-32 levels in GPA were elevated. IL-32 concentrations correlated positively with antiproteinase
3 - ANCA (PR3-ANCA) levels in GPA (P < 0.0001), and with anti-myeloperoxidase ANCA (MPO-ANCA)
inMPA (P = 0.049). IL-32 levels correlated positively with disease activity in GPA andMPA (P < 0.0001). GPA patients
with pulmonary, cutaneous, and musculoskeletal involvement presented the highest IL-6 serum levels. Cutaneous
manifestations correlated positively with IL-6 levels in MPA patients (P = 0.05). ANCA-positive patients with GPA
expressed significantly high IL-6 levels (P = 0.036). No significant difference in IL-32 values was observed between
ANCA-positive and ANCA-negative patients.Conclusions: Patients with GPA andMPA present higher serum IL-32 and
IL-6 levels than controls. IL-32 levels correlate positively with disease activity.
KW  - granulomatosis with polyangiitis
KW  -  interleukin-6
KW  -  interleukin-32
KW  -  microscopic polyangiitis
KW  -  vasculitis
KW  -  ANCA

DO  - 10.1684/ecn.2019.0439