@Article{ecn.2022.0482,
AUTHOR = {Fatemeh Bayati, Maryam Valadi, Armin Ahmadi, Farangis Najaf, Bita Ansaripour, Ehsan Sharif-Paghaleh},
TITLE = {Evaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells},
JOURNAL = {European Cytokine Network},
VOLUME = {33},
YEAR = {2022},
NUMBER = {3},
PAGES = {70--78},
URL = {http://www.techscience.com/ECN/v33n3/65050},
ISSN = {1952-4005},
ABSTRACT = {Although mesenchymal stem cells (MSCs) have exhibited promising immunomodulatory potential in preclinical studies, clinical studies have revealed variable results. These results often depend on environmental cues. Pre-conditioning MSCs with cytokines is one of the methods used to enhance their immunomodulatory effects. In this study, we harvested adipose-derived MSCs from mice and cultured them with different doses of the cytokine, IFN-γ, and the corticosteroid drug, dexamethasone, in order to investigate their effects on MSC immunosuppressive function. We found the co-culture or supernatant of MSCs, pre-conditioned with IFN-γ, together with spleen mononuclear cells resulted in a significant reduction of mononuclear cell proliferation. Although the supernatant of MSCs, pre-conditioned with dexamethasone, showed similar results, dexamethasone pre-conditioning of co-cultured MSCs increased mononuclear cell proliferation. The results further our understanding of immune-related effects of MSCs which may provide a basis for further <i>in vivo</i> studies to achieve better clinical results. We propose that pre-conditioning with cytokines might be an effective method to boost the immunomodulatory effects of MSCs.},
DOI = {10.1684/ecn.2022.0482}
}