TY  - EJOU
AU  - Zhuang, Wei 
AU  - Li, Zhen 

TI  - Antibody targeting TSLP suppresses DSS-induced colitis and activation  of the JAK2/STAT5 pathway in mice
T2  - European Cytokine Network

PY  - 2023
VL  - 34
IS  - 4
SN  - 1952-4005

AB  - <i>Background: </i>There is currently no safe or effective treatment for inflammatory bowel disease (IBD), which is defined as recurrent and persistent intestinal inflammation. Thymic stromal lymphopoietin (TSLP) has been shown to be associated with the pathogenesis of IBD, and the JAK2/STAT5 signalling pathway has demonstrated much promise as a novel therapeutic target for IBD. <i>Materials and Methods:</i> In this study, we first evaluated levels of TSLP in dextran sodium sulphate (DSS)-induced IBD mice. Second, we applied tezepelumab, an anti-TSLP monoclonal antibody (20 µg per mouse, intraperitoneally), to DSS-induced IBD mice and quantified the signs of histopathological change, intestinal inflammation, whereas integrity of the mucosal barrier. In addition, the effect of DSS and/or tezepelumab on the phosphorylation of the JAK/STAT pathway was investigated. <i>Results:</i> TSLP expression levels were elevated in DSS-induced IBD mice, whereas TSLP antibody treatment suppressed the pathological features associated with IBD and alleviated intestinal inflammation and mucosal barrier disruption. Moreover, level of phosphorylated JAK2/STAT5 were increased in DSS-induced IBD mice, but were strongly decreased in the presence of tezepelumab. <i>Conclusions:</i> Our findings suggest that targeting TSLP via the JAK2/STAT5 signalling pathway may be an effective approach for the treatment of IBD.
KW  - inflammatory bowel disease
KW  -  thymic stromal lymphopoietin
KW  -  inflammation
KW  -  mucosal barrier
KW  -  JAK2/ STAT5 pathway

DO  - 10.1684/ecn.2023.0489