TY  - EJOU
AU  - Kim, Kyeong-Min 
AU  - Kim, A-Rang 
AU  - Jang, Won-Gu 

TI  - Linalool Promotes Osteoblast Differentiation via SELENBP1-Dependent Signaling in MC3T3-E1 and C3H10T1/2 Cells
T2  - BIOCELL

PY  - 
VL  - 
IS  - 
SN  - 1667-5746

AB  - <b>Background:</b> Linalool is a monoterpene alcohol with known anti-inflammatory and antioxidant properties, but its role in osteoblast differentiation remains unclear. This study aimed to investigate the osteogenic potential of linalool and to examine the role of selenium-binding protein 1 (Selenbp1) in mediating its effects. <b>Methods:</b> Murine MC3T3-E1 and C3H10T1/2 cells were treated with linalool under osteogenic conditions. Osteoblast differentiation was assessed by alkaline phosphatase (ALP) activity, Alizarin Red S staining, and expression of runt-related transcription factor 2 (Runx2) and distal-less homeobox 5 (Dlx5). The involvement of Selenbp1 was examined using siRNA knockdown and plasmid overexpression. A zebrafish caudal fin regeneration model was used to evaluate <i>in vivo</i> relevance. <b>Results:</b> Linalool significantly enhanced osteoblast differentiation, as evidenced by increased ALP activity (approximately 3–4-fold vs. control, <i>p</i> &lt; 0.01) and matrix mineralization (approximately 2.5–4-fold increase, <i>p</i> &lt; 0.01). The osteogenic transcription factors Runx2 and Dlx5 were significantly upregulated following linalool treatment (<i>p</i> &lt; 0.01). Linalool also markedly induced Selenbp1 expression, showing an approximately 4–5-fold increase in MC3T3-E1 cells and an approximately 8–9-fold increase in C3H10T1/2 cells (<i>p</i> &lt; 0.01). Silencing Selenbp1 attenuated the linalool-induced upregulation of osteogenic markers, whereas its overexpression restored marker expression and enhanced cellular responsiveness to linalool. <i>In vivo</i>, linalool significantly increased zebrafish caudal fin regeneration by approximately 30–45% compared with the vehicle control (<i>p</i> &lt; 0.001). <b>Conclusion:</b> Linalool promotes SELENBP1-dependent osteoblast differentiation <i>in vitro</i> and enhances caudal fin regeneration <i>in vivo</i>, although the involvement of SELENBP1 in the latter was not examined.
KW  - Linalool; selenium-binding protein 1; preosteoblast; mesenchymal stem cell; osteoblast differentiation

DO  - 10.32604/biocell.2026.077163