@Article{biocell.2002.26.263,
AUTHOR = {MARÍA TERESA PONCE, MÓNICA ELIZABETH GUIÑAZÚ, RICARDO TIZIO},
TITLE = {Brief Note : Improved <i>in vitro</i> embryo development of stenospermic grape by putrescine},
JOURNAL = {BIOCELL},
VOLUME = {26},
YEAR = {2002},
NUMBER = {2},
PAGES = {263--266},
URL = {http://www.techscience.com/biocell/v26n2/34001},
ISSN = {1667-5746},
ABSTRACT = {The goal of this study was to determine the effect of putrescine, added to the culture medium, on the <i>in vitro</i> development of stenospermic grape (<i>Vitis vinifera L</i>) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed’s culture medium. In Perlón x Argentina, 2mM of putrescine statistically increased the percentage of total embryos, direct germination, polyembryos and normal plants. In Perlón x G.C88552, only 2 mM of putrescine increased all the variables considered, eventually tripling the percentage of normal plants obtained. The results suggest that the endogenous concentration of putrescine may be a growth limiting factor. Adding putrescine to the culture medium of immature grape seeds is a legitimate resource to significantly increase the results of this technique.},
DOI = {10.32604/biocell.2002.26.263}
}