@Article{biocell.2002.26.217,
AUTHOR = {MARIANA N. MELCHIORRE, HERNÁN R. LASCANO, VICTORIO S.TRIPPI},
TITLE = {Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment},
JOURNAL = {BIOCELL},
VOLUME = {26},
YEAR = {2002},
NUMBER = {2},
PAGES = {217--223},
URL = {http://www.techscience.com/biocell/v26n2/34009},
ISSN = {1667-5746},
ABSTRACT = {Wheat (<i>Triticum aestivum</i>) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene β-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by β-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which “model” cultivar was used.},
DOI = {10.32604/biocell.2002.26.217}
}