TY  - EJOU
AU  - S.TRIPPI, MARIANA N. MELCHIORRE, HERNÁN R. LASCANO, VICTORIO 

TI  - Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment
T2  - BIOCELL

PY  - 2002
VL  - 26
IS  - 2
SN  - 1667-5746

AB  - Wheat (<i>Triticum aestivum</i>) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene β-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by β-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which “model” cultivar was used.
KW  - BAR
KW  -  bombardment
KW  -  GUS
KW  -  transformation
KW  -  <i>Triticum aestivum</i>

DO  - 10.32604/biocell.2002.26.217