TY  - EJOU
AU  - Stoppani**, Lidia M. Lopez*, Marta Dubin**, Patricia H. Carrizo**, Mario H. Burgos***, Amanda Pellegrino De Iraldi*, Andrés O. M. 

TI  - Apoptogenic effect of the lipophilic <i>o</i>-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies
T2  - BIOCELL

PY  - 2003
VL  - 27
IS  - 2
SN  - 1667-5746

AB  - CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran- 5,6-dione; CG-NQ), a β-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; and (h) production of hydrogen peroxide. Expression of such effects varied according to hepatocyte samples and taken together strongly support an apoptotic action of CG-NQ dependent on “reactive oxygen species”. 
KW  - Apoptosis; <i>o</i>-naphthoquinones; hepatocytes; chromatin alteration; mitochondrial swelling membrane; blebs

DO  - 10.32604/biocell.2003.27.213