TY  - EJOU
AU  - Pacheco*, Marcelo de Castro Pazos*, Cristina Pacheco-Soares*, Newton Soares da Silva*, R.A. DaMatta**, M.T.T. 

TI  - Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation
T2  - BIOCELL

PY  - 2003
VL  - 27
IS  - 3
SN  - 1667-5746

AB  - The effects of Photodynamic Therapy using 2<sup>nd</sup> generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2<sup>nd</sup> generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/ cm<sup>2</sup> (Power=26mW; λ=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS<sub>4</sub>) photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line. 
KW  - Photodynamic therapy
KW  -  phthalocyanines
KW  -  laser
KW  -  cell death
KW  -  ultrastructure

DO  - 10.32604/biocell.2003.27.301