@Article{biocell.2003.27.311,
AUTHOR = {LUIS A. CARO, NATALIA SANTECCHIA, PABLO A. MARINANGELI, NÉSTOR R. CURVETTO, LUIS F. HERNÁNDEZ},
TITLE = {<i>Agrobacterium rhizogenes</i> vs auxinic induction for <i>in vitro</i> rhizogenesis of <i>Prosopis chilensis</i> and <i>Nothofagus alpina</i>},
JOURNAL = {BIOCELL},
VOLUME = {27},
YEAR = {2003},
NUMBER = {3},
PAGES = {311--318},
URL = {http://www.techscience.com/biocell/v27n3/37633},
ISSN = {1667-5746},
ABSTRACT = {The induction and improvement of <i>in vitro</i> rhizogenesis of microshoots of <i>Prosopis chilensis</i> (Mol.) Stuntz and <i>Nothofagus alpina</i> (Poep. <i>et</i> Endl. Oerst.) were compared using <i>Agrobacterium rhizogenes</i> (Ar) <i>versus</i> indole-3-butyric acid (IBA) in the culture media. Microshoots of <i>P. chilensis</i> (1-2 cm length), coming from <i>in vitro</i> grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L<sup>-1</sup> benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For <i>N. alpina</i>, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned medium, but with 0.15 mg.L<sup>-1</sup> of BAP. After 2 months, microshoots with 2-3 leaves were selected and cultured in BTMm-agar, supplemented with 5 mg.L<sup>-1</sup> IBA or in liquid BTMm on perlite and, in the presence or absence of <i>A. rhizogenes</i> (Ar) and in combination with 3 mg.L<sup>-1</sup> IBA. Rooting in <i>P. chilensis</i> reached 100.0% when Ar infection was produced in the presence of IBA, increasing both, the number and dry weight of roots. In <i>N. alpina</i>, 90.0% of rooting efficiency was obtained when Ar infection was produced in liquid culture and in the absence of auxin.},
DOI = {10.32604/biocell.2003.27.311}
}