TY  - EJOU
AU  - DEBATTISTA, MARÍA DE LOS ANGELES ALVAREZ, VALERIA E. P. ZARELLI, NORA B.PAPPANO, NORA B. 

TI  - Bacteriostatic action of synthetic polyhydroxylated chalcones against <i>Escherichia coli</i>
T2  - BIOCELL

PY  - 2004
VL  - 28
IS  - 1
SN  - 1667-5746

AB  - In previous work the bacteriostatic action of trihydroxylated chalcones against <i>Staphylococcus aureus</i> ATCC 25 923 was investigated. In this work the action of 2´, 4´, 2-(OH)<sub>3</sub> -chalcone, 2´, 4´, 3-(OH)<sub>3<sup>-</sup></sub> chalcone and 2´, 4´, 4-(OH)<sub>3</sub> –chalcone against <i>Escherichia coli</i> ATCC 25 922 was evaluated. Growth kinetic curves of <i>E.coli</i> were made in nutritive broth added with increasing drug concentrations. The specific growth rates of the microorganisms were calculated by a kinetic turbidimetric method, which was previously probed and the minimal inhibitory concentrations (MIC´s) were evaluated by a mechanism of action proposed. The MICs of 2´, 4´, 3-(OH)<sub>3</sub> -chalcone and 2´, 4´, 2-(OH)<sub>3</sub> -chalcone were 46 µg/ml and 122 µg/ml, respectively. The 2´, 4´, 4-(OH)<sub>3</sub> –chalcone was inactive. The MIC value of 2´, 4´, 3-(OH)<sub>3</sub>-chalcone (46 µg/ml), more active than 2´, 3-(OH)<sub>2</sub>-chalcone (72.2 µg/ml) may be due to the introduction of an electron donating group (-OH) at position 4´ in the aromatic A- ring, which activates the region that includes the 2´-hydroxyl neighbur group and the α,β- unsaturated carbonyl group.
KW  - chalcones
KW  -  bacteriostatic activity
KW  -  hydroxylation

DO  - 10.32604/biocell.2004.28.031