@Article{biocell.2005.29.183,
AUTHOR = {M.T. TERUEL, R.C. CATALANO, J.A. CABODEVILA, S.S. CALLEJAS},
TITLE = {Regulation of mouse embryo development by autocrine throphic factors},
JOURNAL = {BIOCELL},
VOLUME = {29},
YEAR = {2005},
NUMBER = {2},
PAGES = {183--186},
URL = {http://www.techscience.com/biocell/v29n2/37667},
ISSN = {1667-5746},
ABSTRACT = {Embryo development depends on maternal and embryonic factors. When occurs <i>in vitro</i>, embryos secrete factors that stimulate their development. The purpose of this study was to investigate the possible effects of embryos at morula stage on mouse embryo development <i>in vitro</i>. To obtain conditioned media (CM), morulas were cultured in groups of 5 (CM5) or 10 (CM10) in microdrops of Ham-F10 culture medium during 24 h and later they were removed. Subsequently, 365 morulas were cultured in CM5 and CM10 or in Ham-F10 media (as control group). No differences in blastocyst formation could be found between embryos cultured for 24h in Ham-F10, CM5 or CM10 (49.66, 53.04, 60.00% respectively). However, CM5 significantly increased differentiation in embryos cultured for 48h as compared to Ham-F10 medium (80.00% and 64.14 respectively). The CM5 caused a significant increase in the hatching rate compared to Ham-F10 evaluated at 78 and 96 h of culture (66.96 vs. 52.41% and 70.43 vs. 55.17%, respectively). After 72, 78 and 96h of culture the hatching rate for embryos cultured in CM10 was significantly higher than that in Ham-F10 (64.76 vs. 47.59%, 67.62 vs. 52.41% and 73.33 vs. 55.17%, respectively). At 48h of culture, differences between CM5, CM10 and Ham-F10 were not observed. These results suggest that preimplantational mouse embryos produce trophic factor/factors that enhance the differentiation and hatching process.},
DOI = {10.32604/biocell.2005.29.183}
}