TY  - EJOU
AU  - CHEN, ZHONGHAI 
AU  - PANG, YONGZHEN 
AU  - LIU, XIAOJUN 
AU  - WANG, XINGLONG 
AU  - DENG, ZHONGXIANG 
AU  - SUN, XIAOFEN 
AU  - TANG, KEXUAN 

TI  - Molecular cloning and characterization of a novel mannosebinding lectin cDNA from <i>Zantedeschia aethiopica</i>
T2  - BIOCELL

PY  - 2005
VL  - 29
IS  - 2
SN  - 1667-5746

AB  - Using RNA extracted from <i>Zantedeschia aethiopica</i> young leaves and primers designed according to the conservative regions of <i>Araceae</i> lectins, the full-length cDNA of <i>Z. aethiopica</i> agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of <i>zaa</i> was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of <i>zaa</i> gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had many common characters of mannose-binding lectin superfamily and ZAA was a mannose-binding lectin with three mannose-binding sites. Southern blot analysis of the genomic DNA revealed that <i>zaa</i> belonged to a multi-copy gene family.
KW  - Mannose-binding lectin
KW  -  RACE
KW  -  <i>Zantedeschia aethiopica</i>
KW  -  ZAA

DO  - 10.32604/biocell.2005.29.187