@Article{biocell.2005.29.313, AUTHOR = {A. C. PONTAROLI, E. L. CAMADRO}, TITLE = {Brief Note : Plant regeneration after long term callus culture in clones of Asparagus officinalis L.*}, JOURNAL = {BIOCELL}, VOLUME = {29}, YEAR = {2005}, NUMBER = {3}, PAGES = {313--317}, URL = {http://www.techscience.com/biocell/v29n3/37680}, ISSN = {1667-5746}, ABSTRACT = {Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l-1 2,4-D and 1 mg.l-1 kinetin. Shoot primordia (SP) induction from >18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone ‘265’ on MS medium with 5 mg.l-1 2iP, 1 mg.l-1 IAA, 3% sucrose and 0.9% agar. The highest percentage of root induction (100%) was achieved with clone ‘265’ on MS medium with 0.1 mg.l-1 kinetin, 0.1 mg.l-1 NAA, 1.32 mg.l-1 ancymidol, 7% glucose and 0.8% agar. Important medium x genotype interactions were detected, pointing to the need of adjusting this and other in vitro protocols for specific asparagus genotypes.}, DOI = {10.32604/biocell.2005.29.313} }