@Article{biocell.2005.29.313,
AUTHOR = {A. C. PONTAROLI, E. L. CAMADRO},
TITLE = {Brief Note : Plant regeneration after long term callus culture in clones of <i>Asparagus officinalis</i> L.*},
JOURNAL = {BIOCELL},
VOLUME = {29},
YEAR = {2005},
NUMBER = {3},
PAGES = {313--317},
URL = {http://www.techscience.com/biocell/v29n3/37680},
ISSN = {1667-5746},
ABSTRACT = {Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of <i>Asparagus officinalis</i> cv. Argenteuil, to establish a suitable protocol for a prospective <i>in vitro</i> selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l<sup>-1</sup> kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l<sup>-1</sup> 2,4-D and 1 mg.l<sup>-1</sup> kinetin. Shoot primordia (SP) induction from >18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone ‘265’ on MS medium with 5 mg.l<sup>-1</sup> 2iP, 1 mg.l<sup>-1</sup> IAA, 3% sucrose and 0.9% agar. The highest percentage of root induction (100%) was achieved with clone ‘265’ on MS medium with 0.1 mg.l<sup>-1</sup> kinetin, 0.1 mg.l<sup>-1</sup> NAA, 1.32 mg.l<sup>-1</sup> ancymidol, 7% glucose and 0.8% agar. Important medium x genotype interactions were detected, pointing to the need of adjusting this and other <i>in vitro</i> protocols for specific asparagus genotypes.},
DOI = {10.32604/biocell.2005.29.313}
}