@Article{biocell.2006.30.015,
AUTHOR = {JUAN LIN, XUANWEI ZHOU, JIONG FEI, ZHIHUA LIAO, WANG JIN, XIAOFEN SUN, KEXUAN TANG},
TITLE = {Genomic cloning and characterization of a PPA gene encoding a mannose-binding lectin from <i>Pinellia pedatisecta</i>},
JOURNAL = {BIOCELL},
VOLUME = {30},
YEAR = {2006},
NUMBER = {1},
PAGES = {15--25},
URL = {http://www.techscience.com/biocell/v30n1/37683},
ISSN = {1667-5746},
ABSTRACT = {A gene encoding a mannose-binding lectin, <i>Pinellia pedatisecta</i> agglutinin (PPA), was isolated from leaves of <i>Pinellia pedatisecta</i> using genomic walker technology. The <i>ppa</i> contained an 1140-bp 5’-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3’-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5’-upstream region of <i>ppa</i>. The <i>ppa</i> showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other <i>Araceae</i> species such as <i>Pinellia ternata</i>, <i>Arisaema heterophyllum</i>, Colocasia esculenta and <i>Arum maculatum</i>. At the amino acid level, PPA also shared varying homology (ranging from 40% to 85%) with mannose-binding lectins from other plant species, such as those from <i>Araceae, Alliaceae, Iridaceae, Lillaceae, Amaryllidaceae</i> and <i>Bromeliaceae</i>. The cloning of the <i>ppa</i> gene not only provides a basis for further investigation of PPA’s structure, expression and regulation mechanism, but also enables us to test its potential role in controlling pests and fungal diseases by transferring the gene into tobacco and rice in the future.},
DOI = {10.32604/biocell.2006.30.015}
}