TY - EJOU
AU - NOME, CLAUDIA FERNANDA
AU - LAGUNA, IRMA GRACIELA
TI - Localization of Sweet potato chlorotic stunt virus (SPCSV) in synergic infection with Potyviruses in sweet potato
T2 - BIOCELL
PY - 2007
VL - 31
IS - 1
SN - 1667-5746
AB - Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one
of the most frequent is the Sweet potato virus disease (SPVD), caused by sweet potato chlorotic stunt virus
(SPCSV) and sweet potato feathery mottle virus (SPFMV) co-infection. In Argentina, there exists the sweet
potato chlorotic dwarf (SPCD), a sweet potato disease caused by triple co-infection with SPCSV, SPFMV and
sweet potato mild speckling virus(SPMSV). Both diseases cause a synergism between the potyviruses(SPFMV
and SPMSV) and the crinivirus (SPCSV). Up to date, studies carried out on the interaction among these
three viruses have not described their localization in the infected tissues. In single infections, virions of the
crinivirus genus are limited to the phloem while potyviral virions are found in most tissues of the infected
plant.
The purpose of this work was to localize the heat shock protein 70 homolog (HSP70h), a movement
protein for genus crinivirus, of an Argentinean SPCSV isolate in its single infection and in its double and
triple co-infection with SPFMV and SPMSV. The localization was made by in situ hybridization (ISH) for
electron microscopy (EM) on ultrathin sections of sweet potato cv. Morada INTA infected tissues.
The results demonstrated that viral RNA coding HSP70h is restricted to phloem cells during crinivirus
single infection, while it was detected outside the phloem in infections combined with the potyviruses involved in chlorotic dwarf disease.
KW - Sweet potato chlorotic stunt virus
KW - HSP70h
KW - in situ hybridization
KW - crinivirus
KW - sweet potato
DO - 10.32604/biocell.2007.31.023