TY  - EJOU
AU  - ERGEN, KIVANÇ 
AU  - BEKTAŞ, MUHAMMET 
AU  - GÖKÇE, SINA 
AU  - NURTEN, RÜSTEM 

TI  - Endogenous ADP-ribosylation of eukaryotic elongation factor 2 and its 32 kDa tryptic fragment
T2  - BIOCELL

PY  - 2007
VL  - 31
IS  - 1
SN  - 1667-5746

AB  -  Eukaryotic elongation factor 2 (eEF-2) can undergo ADP-ribosylation in the absence of diphtheria toxin. The binding of free ADP-ribose and endogenous transferase-dependent ADP-ribosylation were
distinct reactions for eEF-2, as indicated by different findings. Incubation of eEF-2 tryptic fragment 32/33
kDa (32F) with NAD was ADP-ribosylated and gave rise to the covalent binding of ADP-ribose to eEF-2. 32F
was revealed to be at the C-terminal by Edman degradation sequence analysis.<br/>
In our study, the elution of 32F from SDS-PAGE was ADP-ribosylated both in the presence and absence of
diphtheria toxin. These results suggest that endogenous ADP-ribosylation of 32F might be related to protein
synthesis. This modification appears to be important for the cell function.
KW  - Eukaryotic elongation factor 2
KW  -  endogenous ADP-ribosylation
KW  -  protein synthesis
KW  -  trypsin digestion
KW  -  diphtheria toxin

DO  - 10.32604/biocell.2007.31.061