@Article{biocell.2007.31.365,
AUTHOR = {MARÍA ESTHER DIUPOTEX-CHONG, NÉSTOR J. CAZZANIGA, MANUEL URIBE-ALCOCER},
TITLE = {Karyological and electrophoretic differences between <i>Pomacea flagellata</i> and <i>P. patula catemacensis</i> (Caenogastropoda: Ampullariidae)},
JOURNAL = {BIOCELL},
VOLUME = {31},
YEAR = {2007},
NUMBER = {3},
PAGES = {365--373},
URL = {http://www.techscience.com/biocell/v31n3/33985},
ISSN = {1667-5746},
ABSTRACT = {The widespread Mexican apple snail <i>Pomacea flagellata</i> (Say 1827) and the strictly endemic
"tegogolo" <i>P. patula catemacensis</i> (Baker 1922) (restricted to Lake Catemaco), are the only known American
Ampullariidae that have haploid complements n=13. <i>Pomacea patula catemacensis</i> has suffered a critical
reduction in abundance due to immoderate fishing for human consumption. Chromosome slides were obtained from colchicine-injected <i>Pomacea</i> snails collected from nine locations along the coastal zone of the
Gulf of Mexico, including Lake Catemaco, for use in principal component analysis (PCA). Total proteins in
foot homogenates were analyzed through isoelectric focusing (IEF) and native-PAGE electrophoresis on polyacrylamide gels. The chromosome number 2n=26 was confirmed for snails from all locations, with a uniform
9 m + 4 sm formula. However, <i>P. patula catemacensis</i> showed significantly larger chromosomes (absolute
size) than any population of <i>P. flagellata. Pomacea patula catemacensis</i> also differed from all populations of
<i>P. flagellata</i> in a PCA with standardized data, i.e., independently of the absolute size difference between
species. Proteins with an acid isoelectric point were dominant in the foot of both species. The electrophoresis
analysis showed that <i>P. flagellata</i> has 17 protein bands, with an upper bound at IEF=7.6, while <i>P. patula
catemacensis</i> has only 15 bands, with an upper bound at IEF=7 and a more evenly spaced band pattern.
Molecular weights ranged from 40 to approximately 130 kDa in both species. Proteins with high values (>94
kDa) were the most abundant. <i>Pomacea patula catemacensis</i> showed a band of 93 kDa, which was absent
from all specimens of <i>P. flagellata</i>. Samples of <i>P. flagellata</i> did not cluster according to any geographical
pattern in the statistical analyses, nor did they show any taxonomically useful differences in their electrophoretic patterns that merit sub-specific discrimination.},
DOI = {10.32604/biocell.2007.31.365}
}