@Article{biocell.2008.32.001,
AUTHOR = {FRANCISCO CAPANI, EZEQUIEL SARACENO, VALERIA ROMINA BOTI, LAURA AON-BERTOLINO, JUAN CARLOS FERNÁNDEZ, FERNANDO GATO, MARIA SOL KRAUSE, LISANDRO GIRALDEZ, MARK H. ELLISMAN, HÉCTOR COIRINI},
TITLE = {Review : A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation},
JOURNAL = {BIOCELL},
VOLUME = {32},
YEAR = {2008},
NUMBER = {1},
PAGES = {1--8},
URL = {http://www.techscience.com/biocell/v32n1/37728},
ISSN = {1667-5746},
ABSTRACT = {Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions.},
DOI = {10.32604/biocell.2008.32.001}
}