TY - EJOU AU - CAPANI, FRANCISCO AU - SARACENO, EZEQUIEL AU - BOTI, VALERIA ROMINA AU - AON-BERTOLINO, LAURA AU - FERNÁNDEZ, JUAN CARLOS AU - GATO, FERNANDO AU - KRAUSE, MARIA SOL AU - GIRALDEZ, LISANDRO AU - ELLISMAN, MARK H. AU - COIRINI, HÉCTOR TI - Review : A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation T2 - BIOCELL PY - 2008 VL - 32 IS - 1 SN - 1667-5746 AB - Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions. KW - F-actin KW - phalloidin-eosin KW - phootoxidation KW - 3-D reconstructions KW - electron tomography DO - 10.32604/biocell.2008.32.001