TY  - EJOU
AU  - SEONG, SAMIN HONG, KYOUNGSOO PARK, CHAN YUN KIM, GONG JE 

TI  - Short Communication: Agmatine inhibits hypoxia-induced TNF-α release from cultured retinal ganglion cells
T2  - BIOCELL

PY  - 2008
VL  - 32
IS  - 2
SN  - 1667-5746

AB  - The effect of hypoxia on the release of tumor necrosis factor-α (TNF-α) in transformed rat retinal ganglion cells (RGCs) and the effect of agmatine on the hypoxia-induced production of TNF-α in RGCs were evaluated. RGCs were cultured under hypoxic conditions with 5% oxygen, with or without 100 μM agmatine. The expression levels of TNF-α and its receptor-1 (TNF-R1) were investigated by Western blot analysis. After 6 hours of hypoxia, we noted an increase in TNF-α production in RGCs. Agmatine significantly reduced TNF-α level after 12 hours of hypoxic treatment. The expression of TNF-R1 was not affected by the hypoxia or agmatine treatment. Our results show that agmatine inhibits the TNF-α production of RGCs in hypoxic condition. These results demonstrate a possible neuroprotective mechanism for agmatine against hypoxic damage in RGCs.
KW  - retinal ganglion cell
KW  -  tumor necrosis factor-α
KW  -  hypoxia
KW  -  agmatine

DO  - 10.32604/biocell.2008.32.201