TY  - EJOU
AU  - LIN, JUAN 
AU  - ZHANG, WEN 
AU  - SHI, MINGZHU 
AU  - WANG, XINGLONG 
AU  - SUN, XIAOFEN 
AU  - TANG, KEXUAN 

TI  - Isolation and molecular characterization of a <i>cax</i> gene from <i>Capsella bursa-pastoris</i>
T2  - BIOCELL

PY  - 2008
VL  - 32
IS  - 3
SN  - 1667-5746

AB  - A new cation exchangers (CAXs) gene was cloned and characterized from <i>Capsella bursapastoris</i> by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of <i>cax</i> from <i>C. bursa-pastoris</i> (designated as <i>Cbcax51</i>) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na<sup>+</sup>/Ca<sup>2+</sup> exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that <i>Cbcax51</i> could be activated by cold triggering and was related to the cold acclimation process, but its expression is regulated negatively by drought and not affected by ABA or salt.
KW  - <i>Capsella bursa-pastoris</i>
KW  -  cation exchangers (CAXs)
KW  -  RACE
KW  -  freezing tolerance

DO  - 10.32604/biocell.2008.32.229