TY  - EJOU
AU  - NAGATSUKA*, ANDREA P. RODRIGUEZ, HIDETSUGU TSUJIGIWA, MEHMET GUNDUZ, BEYHAN CENGIZ, NORIYUKI NAGAI, RYO TAMAMURA, SILVIA S. BORKOSKY, TOHRU TAKAGI, MIHO INOUE, HITOSHI 

TI  - Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells
T2  - BIOCELL

PY  - 2009
VL  - 33
IS  - 1
SN  - 1667-5746

AB  - Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both <i>in vitro</i> cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein <i>in vivo</i>. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines <i>in vitro</i>. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells <i>in vivo</i>.
KW  - MDPC-23 cells
KW  -  KUSA/A1 cells
KW  -  diffusion chamber
KW  -  RT-PCR analysis
KW  -  immunohistochemical analysis

DO  - 10.32604/biocell.2009.33.039