TY  - EJOU
AU  - VILLALOBOS, VIRGINIA 
AU  - BONILLA, ERNESTO 
AU  - CASTELLANO, ALAN 
AU  - NOVO, ERNESTO 
AU  - CASPERSEN, RALPH 
AU  - GIRALDOTH, DEBORA 
AU  - MEDINA-LEENDERTZ, SHIRLEY 

TI  - Ultrastructural changes of the olfactory bulb in manganesetreated mice
T2  - BIOCELL

PY  - 2009
VL  - 33
IS  - 3
SN  - 1667-5746

AB  - The effect of manganese toxicity on the ultrastructure of the olfactory bulb was evaluated. Male albino mice were injected intraperitoneally with MnCl<sub>2</sub> (5 mg/Kg/day) five days per week during nine weeks. The control group received NaCl (0.9%). The olfactory bulbs of five mice from each group were processed for transmission electron microscopy after 2, 4, 6 and 9 weeks of manganese treatment. On week 2, some disorganization of the myelin sheaths was observed. After 4 weeks, degenerated neurons with dilated cisternae of rough endoplasmic reticulum and swollen mitochondria appeared. A certain degree of gliosis with a predominance of astrocytes with swollen mitochondria, disorganization of the endomembrane system, dilation of the perinuclear cisternae and irregularly shaped nuclei with abnormal chromatin distribution were observed after 6 weeks. Some glial cells showed disorganization of the Golgi apparatus. On week 9, an increase in the number of astrocytes, whose mitochondrial cristae were partially or totally erased, and a dilation of the rough endoplasmic reticulum were found. Neurons appear degenerated, with swollen mitochondria and a vacuolated, electron dense cytoplasm. These changes seem to indicate that the olfactory bulb is sensitive to the toxic effects of manganese.
KW  - Metal toxicity
KW  -  toxicity
KW  -  neurodegeneration
KW  -  electron microscopy

DO  - 10.32604/biocell.2009.33.187