@Article{biocell.2010.34.071,
AUTHOR = {CHRIS VORSTER, ANNIE JOUBERT},
TITLE = {<i>In vitro</i> effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line},
JOURNAL = {BIOCELL},
VOLUME = {34},
YEAR = {2010},
NUMBER = {2},
PAGES = {71--80},
URL = {http://www.techscience.com/biocell/v34n2/37780},
ISSN = {1667-5746},
ABSTRACT = {In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM’s <i>in vitro</i> efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4μM. In addition, mitotic indices revealed that 2ME-BM induces a G<sub>2</sub>M block. The latter was confirmed by flow cytometric analyses where increased sub-G<sub>1</sub> and G<sub>2</sub> /M fractions, as well as an increase in cyclin B1 levels were observed. Further <i>in vitro</i> research into the mechanism of this potentially useful anticancer compound is thus warranted.},
DOI = {10.32604/biocell.2010.34.071}
}