@Article{biocell.2010.34.139,
AUTHOR = {YAN PI1, KEJI JIANG2, RONG HOU, YIFU GONG, JUAN LIN, XIAOFEN SUN, AND KEXUAN TANG},
TITLE = {Examination of camptothecin and 10-hydroxycamptothecin in <i>Camptotheca acuminata</i> plant and cell culture, and the affected yields under several cell culture treatments},
JOURNAL = {BIOCELL},
VOLUME = {34},
YEAR = {2010},
NUMBER = {3},
PAGES = {139--144},
URL = {http://www.techscience.com/biocell/v34n3/37788},
ISSN = {1667-5746},
ABSTRACT = {Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from <i>Camptotheca acuminata</i>, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10- hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of <i>Camptotheca acuminata</i> cells in culture did not correlate with contents of camptothecin and 10-hydroxycamptothecin. Alkaloid accumulation by cells under various treatments (heavy metal ions, UV-B), methyl-jasmonate, abscisic acid, salicylic acid and hydrogen peroxide was examined, and the most notable effects appeared in the cells induced by UV-B light (which showed an 11-fold increase in camptothecin concentration) and by salicylic acid (which showed a 25-fold increase in 10-hydroxycamptothecin concentration). These results are significant in the context of the production of both pharmaceuticals.},
DOI = {10.32604/biocell.2010.34.139}
}