@Article{biocell.2011.35.043,
AUTHOR = {YOUNG HWA LEE, HEE SAM NA , SO YEON JEONG, SUNG HEE JEONG , HAE RYOUN PARK , JIN CHUNG},
TITLE = {Comparison of inflammatory microRNA expression in healthy and periodontitis tissues},
JOURNAL = {BIOCELL},
VOLUME = {35},
YEAR = {2011},
NUMBER = {2},
PAGES = {43--50},
URL = {http://www.techscience.com/biocell/v35n2/37233},
ISSN = {1667-5746},
ABSTRACT = {MicroRNAs (miRNAs) are short RNA molecules that negatively regulate gene expression
primarily by degrading target mRNA or inhibit the translation of protein product. Recently, many reports
have shown the altered miRNA expression in various diseases. However, there are no reports on miRNA
expression related to periodontitis. Thus, this study aimed to compare the miRNAs differentially expressed in
healthy and chronic periodontitis tissues and to determine the miRNAs closely associated with chronic periodontitis. To find out the miRNAs differentially induced in healthy and chronic periodontitis tissues, miRNA
microarray was carried out and the expression of miRNAs was confirmed by real-time PCR. According to
miRNA microarray analyses, six miRNA genes, let-7a, let-7c, miR-130a, miR301a, miR-520d, and miR-
548a, were up-regulated more than 8 fold compared to the healthy gingiva. The expression of twenty-two
miRNAs was increased more than 4 fold. Among these miRNAs, eight miRNAs which are known to be
closely related to inflammation were selected. Six of these miRNA genes, miR-181b, miR-19b, miR-23a,
miR-30a, miR-let7a, and miR-301a, were amplified successfully and increased much more in periodontitis
gingivae than in healthy ones. In summary, this study indicate that six miRNAs up-regulated in periodontitis
gingiva may play a key role in chronic periodontitis.},
DOI = {10.32604/biocell.2011.35.043}
}