@Article{biocell.2012.36.031,
AUTHOR = {MAURO RODRIGO SURENCISKI, EDUARDO ALBERTO FLACHSLAND, GRACIELA TERADA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY},
TITLE = {Cryopreservation of <i>Cyrtopodium hatschbachii</i> Pabst (Orchidaceae) immature seeds by encapsulation-dehydration},
JOURNAL = {BIOCELL},
VOLUME = {36},
YEAR = {2012},
NUMBER = {1},
PAGES = {31--36},
URL = {http://www.techscience.com/biocell/v36n1/37793},
ISSN = {1667-5746},
ABSTRACT = {The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of <i>Cyrtopodium hastchbachii</i> Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in <i>Cyrtopodium hatschbachii</i>.},
DOI = {10.32604/biocell.2012.36.031}
}