TY  - EJOU
AU  - SURENCISKI, MAURO RODRIGO 
AU  - FLACHSLAND, EDUARDO ALBERTO 
AU  - TERADA, GRACIELA 
AU  - MROGINSKI, LUIS AMADO 
AU  - REY, HEBE YOLANDA 

TI  - Cryopreservation of <i>Cyrtopodium hatschbachii</i> Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
T2  - BIOCELL

PY  - 2012
VL  - 36
IS  - 1
SN  - 1667-5746

AB  - The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of <i>Cyrtopodium hastchbachii</i> Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in <i>Cyrtopodium hatschbachii</i>.
KW  - Cryoconservation
KW  -  liquid nitrogen
KW  -  calcium alginate
KW  -  activated charcoal
KW  -  sucrose

DO  - 10.32604/biocell.2012.36.031