@Article{biocell.2012.36.047,
AUTHOR = {ISRAEL A. VEGA, FEDERICO A. DELLAGNOLA, JORGE A. HURST, MARTÍN S. GODOY AND ALFREDO CASTRO-VAZQUEZ},
TITLE = {A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail <i>Pomacea canaliculata</i>},
JOURNAL = {BIOCELL},
VOLUME = {36},
YEAR = {2012},
NUMBER = {2},
PAGES = {47--55},
URL = {http://www.techscience.com/biocell/v36n2/37795},
ISSN = {1667-5746},
ABSTRACT = {Pigments present in the brown-greenish C morph of an intracellular endosymbiont of <i>Pomacea canaliculata</i> were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (C<sub>I</sub> , C<sub>II</sub>  and C<sub>III</sub> ), were studied by positive ion fast atom bombardment-mass spectrometry (FAB–MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored C<sub>I</sub> fraction; (2) a mixture of pheophorbides <i>a</i> and <i>b</i> in the major green fraction, C<sub>II</sub>; and (3) a modified pheophorbide a in the smaller green fraction, C<sub>III</sub> . Aqueous extracts of the C endosymbiont did not show evidence of the occurrence of C-phycocyanin, allophycocyanin or phycoerithrin (light absorption, fluorescence emission, and electrophoresis of the protein moieties) while cyanobacterial cells (<i>Nostoc</i> sp.) showed evidence of C-phycocyanin and allophycocyanin. The possible phylogenetic and functional significance of the pigments present in the C endosymbiont is discussed.},
DOI = {10.32604/biocell.2012.36.047}
}