@Article{biocell.2012.36.143,
AUTHOR = {VIVIANE DAL-SOUTO FRESCURA, HAYWOOD DAIL LAUGHINGHOUSE IV, THAIS SCOTTI DO CANTODOROW, SOLANGE BOSIO TEDESCO},
TITLE = {Pollen viability of <i>Polygala paniculata</i> L. (Polygalaceae) using different staining methods},
JOURNAL = {BIOCELL},
VOLUME = {36},
YEAR = {2012},
NUMBER = {3},
PAGES = {143--145},
URL = {http://www.techscience.com/biocell/v36n3/37806},
ISSN = {1667-5746},
ABSTRACT = {<i>Polygala paniculata</i> L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as ‘barba-de-São-João’, ‘barba-de-bode’, ‘vassourinha branca’, and ‘mimosa’. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander’s stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of <i>P. paniculata</i> using the Alexander’s stain, which proved the most adequate method to estimate pollen viability.},
DOI = {10.32604/biocell.2012.36.143}
}