TY  - EJOU
AU  - FRESCURA, VIVIANE DAL-SOUTO 
AU  - IV, HAYWOOD DAIL LAUGHINGHOUSE 
AU  - CANTODOROW, THAIS SCOTTI DO 
AU  - TEDESCO, SOLANGE BOSIO 

TI  - Pollen viability of <i>Polygala paniculata</i> L. (Polygalaceae) using different staining methods
T2  - BIOCELL

PY  - 2012
VL  - 36
IS  - 3
SN  - 1667-5746

AB  - <i>Polygala paniculata</i> L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as ‘barba-de-São-João’, ‘barba-de-bode’, ‘vassourinha branca’, and ‘mimosa’. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander’s stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of <i>P. paniculata</i> using the Alexander’s stain, which proved the most adequate method to estimate pollen viability.
KW  - pollen grains
KW  -  barba-de-bode
KW  -  acetic orcein
KW  -  acetic carmine
KW  -  Alexander’s stain

DO  - 10.32604/biocell.2012.36.143