@Article{biocell.2013.37.001,
AUTHOR = {LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO},
TITLE = {High passage numbers induce resistance to apoptosis in C2C12 muscle cells},
JOURNAL = {BIOCELL},
VOLUME = {37},
YEAR = {2013},
NUMBER = {1},
PAGES = {1--9},
URL = {http://www.techscience.com/biocell/v37n1/35103},
ISSN = {1667-5746},
ABSTRACT = { Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the
present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2
O2
 as a function of
culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA
(mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential,
we observed that cells in the h-C2C12 group were resistant to H2
O2 induction of apoptosis. We propose serially
cultured C2C12 cells as a refractory model to H2
O2
-induced apoptosis. In addition, the data obtained in this
work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.},
DOI = {10.32604/biocell.2013.37.001}
}