TY - EJOU AU - PRONSATO, LUCIA AU - COLLA, ANABELA LA AU - RONDA, ANA CAROLINA AU - MILANESI, LORENA AU - BOLAND, RICARDO AU - VASCONSUELO, ANDREA TI - High passage numbers induce resistance to apoptosis in C2C12 muscle cells T2 - BIOCELL PY - 2013 VL - 37 IS - 1 SN - 1667-5746 AB - Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2 O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2 O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2 O2 -induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells. KW - over-subculturing changes KW - aged muscle cultures KW - mitochondrial DNA DO - 10.32604/biocell.2013.37.001