@Article{biocell.2013.37.055, AUTHOR = {Yi ZOU, Timothy C COX}, TITLE = {A likely role for the PH-domain containing protein, PEPP2/ PLEKHA5, at the membrane-microtubule cytoskeleton interface}, JOURNAL = {BIOCELL}, VOLUME = {37}, YEAR = {2013}, NUMBER = {3}, PAGES = {55--61}, URL = {http://www.techscience.com/biocell/v37n3/37815}, ISSN = {1667-5746}, ABSTRACT = {PH (pleckstrin homology) domains are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular compartments with assistances of alternative binding partners. PH domain-containing proteins have been found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2 (also known as PLEKHA5), displays moderate phosphoinositide binding specificity. Full length PEPP2 was observed to variably associate with both the plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating cells. Overexpression of PEPP2 increased membrane microviscosity, indicating a potential role for PEPP2 in regulating function of microtubule-dependent membrane functions.}, DOI = {10.32604/biocell.2013.37.055} }