TY  - EJOU
AU  - ZOU, Yi 
AU  - COX, Timothy C 

TI  - A likely role for the PH-domain containing protein, PEPP2/ PLEKHA5, at the membrane-microtubule cytoskeleton interface
T2  - BIOCELL

PY  - 2013
VL  - 37
IS  - 3
SN  - 1667-5746

AB  - PH (pleckstrin homology) domains are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular compartments with assistances of alternative binding partners. PH domain-containing proteins have been found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2 (also known as PLEKHA5), displays moderate phosphoinositide binding specificity. Full length PEPP2 was observed to variably associate with both the plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating cells. Overexpression of PEPP2 increased membrane microviscosity, indicating a potential role for PEPP2 in regulating function of microtubule-dependent membrane functions.
KW  - Microtubule
KW  -  membrane
KW  -  phosphoinositide
KW  -  microviscosity
KW  -  PH domain

DO  - 10.32604/biocell.2013.37.055