TY - EJOU
AU - MÖHLINGER, Agustín César
AU - SIFFREDI, Virginia Paula
AU - CHULIBERT, Serenela
AU - PONESSA, Adriana
AU - NOTARIO, Rodolfo David
AU - DLUGOVITZKY, Diana Graciela
TI - Immune strategies of phagoctytic cells stimulated in vitro with live and heat-inactivated Streptococcus pyogenes
T2 - BIOCELL
PY - 2013
VL - 37
IS - 3
SN - 1667-5746
AB - Streptococcus pyogenes (group A Streptococcus) is frequently involved in a wide range of human diseases. Here we evaluated polymorphonuclear neutrophils and mononuclear cells from healthy subjects for their bactericidal function after stimulation with live and inactivated Streptococcus pyogenes (Streptococcus Group A). Mononuclear cells and Neutrophils were isolated from heparinized blood samples (n=18) using a Ficoll-Hypaque gradient and cultured in RPMI 1640 for 18 hours with a suspension of either live or inactivated Streptococcus pyogenes. Both the respiratory burst (flow cytometry) and nitrite, TNF and IL17 production (ELISA) were measured in the cell culture supernatants. An increased respiratory burst (expressed as R index) was induced by both live and inactivated bacteria. Also, increased nitrite, TNF and IL17 concentrations were found in cell culture supernatants in both cases. These findings may provide some explanation as to the roles played by neutrophils and mononuclear cells in Streptococcus pyogenes immunopathogenicity.
KW - Respiratory burst
KW - nitrites
KW - TNFa
KW - IL17
DO - 10.32604/biocell.2013.37.063