@Article{biocell.2013.37.077,
AUTHOR = {Hui ZHAO, Fan ZHANG, Ling LI, Pingang HE, Yuzhi FANG},
TITLE = {Exocytosis determination of SH-SY5Y single cell stimulated by different stimulants on indium tin oxide (ITO) micro-pore electrode},
JOURNAL = {BIOCELL},
VOLUME = {37},
YEAR = {2013},
NUMBER = {3},
PAGES = {77--83},
URL = {http://www.techscience.com/biocell/v37n3/37818},
ISSN = {1667-5746},
ABSTRACT = {The human neuroblastoma SH-SY5Y cell line has been used as a model to study mechanisms of neurotransmitter release. In order to study the mechanism of SH-SY5Y single cell exocytosis stimulated by different stimulants, including high K+, 3-(1-nitroso-2-pyrrolidinyl) pyridine and nicotine, a type of indium tin oxide (ITO) micro-pore electrode was used to obtain the corresponding amperometric response time. When the cell is stimulated by 0.1 M K+ , almost immediate exocytosis could be detected, due to the rapid depolarization of cell membrane. However, the stimulations with 1 mM nicotine and 3-(1-nitroso-2-pyrrolidinyl) pyridine result in a short delay between stimulation and exocytosis, which can be correlated with the time needed for binding of the stimulant to the nicotinic acetylcholine (ACh) receptor and the induction of post-binding phenomena. Thus, the response time of SH-SY5Y single cell exocytosis is significantly affected by the exocytosis mechanisms.},
DOI = {10.32604/biocell.2013.37.077}
}