@Article{biocell.2014.38.017,
AUTHOR = {Yanhong ZHEN, Li HAN, Kailai CAI, Lijun HUO, Hasan RIAZ, Canjie WU, Aixin LIANG , Lei SANG, Liguo YANG},
TITLE = {Overexpression of inhibin α (1-32) fusion protein promotes apoptosis and cell cycle arrest in a cervical cancer cell model (Hela cells)},
JOURNAL = {BIOCELL},
VOLUME = {38},
YEAR = {2014},
NUMBER = {1},
PAGES = {17--24},
URL = {http://www.techscience.com/biocell/v38n1/34065},
ISSN = {1667-5746},
ABSTRACT = {Inhibins play important roles in the reproductive system. To evaluate whether inhibin α (1-32)
fusion protein plays a role in cervical cancer growth, the plasmid pVAX-inhα was constructed and its effect on
proliferation and apoptosis of the human cervical cancer cell line (Hela) was checked by flow cytometry and
real-time PCR. The expression and localization of inhibin α protein were detected by RT-PCR and confocal microscopy which showed that inhibin α protein was expressed and localized in the nucleus of Hela cells. Over expression of inhibin α gene significantly induced cell apoptosis and ceased S phase of cell cycle. Furthermore, cell
proliferation was significantly suppressed 96 h post-transfection and mRNA level of anti-apoptosis genes (Bcl-2,
NFκB) were decreased but pro-apoptosis genes (Bax, wild type p53) and inhibin co receptor (TGFβR3) were
increased, indicating that inhibin, through its co-receptor, might activate apoptotic and cell growth cascades
which regulate proliferation and apoptosis in Hela cells. These results suggest that inhibin α (1-32) fusion protein, located in the cell nucleus, can regulate Hela cells growth and apoptosis by induction of apoptotic pathways
such as NFκB, Bcl-2 and p53 families. These findings may have a significant impact on future research regarding
cervical cancer cell lines},
DOI = {10.32604/biocell.2014.38.017}
}