TY  - EJOU
AU  - ZHEN, Yanhong 
AU  - HAN, Li 
AU  - CAI, Kailai 
AU  - HUO, Lijun 
AU  - RIAZ, Hasan 
AU  - WU, Canjie 
AU  - LIANG, Aixin 
AU  - YANG, Liguo 

TI  - Overexpression of inhibin α (1-32) fusion protein promotes apoptosis and cell cycle arrest in a cervical cancer cell model (Hela cells)
T2  - BIOCELL

PY  - 2014
VL  - 38
IS  - 1
SN  - 1667-5746

AB  - Inhibins play important roles in the reproductive system. To evaluate whether inhibin α (1-32)
fusion protein plays a role in cervical cancer growth, the plasmid pVAX-inhα was constructed and its effect on
proliferation and apoptosis of the human cervical cancer cell line (Hela) was checked by flow cytometry and
real-time PCR. The expression and localization of inhibin α protein were detected by RT-PCR and confocal microscopy which showed that inhibin α protein was expressed and localized in the nucleus of Hela cells. Over expression of inhibin α gene significantly induced cell apoptosis and ceased S phase of cell cycle. Furthermore, cell
proliferation was significantly suppressed 96 h post-transfection and mRNA level of anti-apoptosis genes (Bcl-2,
NFκB) were decreased but pro-apoptosis genes (Bax, wild type p53) and inhibin co receptor (TGFβR3) were
increased, indicating that inhibin, through its co-receptor, might activate apoptotic and cell growth cascades
which regulate proliferation and apoptosis in Hela cells. These results suggest that inhibin α (1-32) fusion protein, located in the cell nucleus, can regulate Hela cells growth and apoptosis by induction of apoptotic pathways
such as NFκB, Bcl-2 and p53 families. These findings may have a significant impact on future research regarding
cervical cancer cell lines
KW  - inhibin α (1-32)
KW  -  Hela cells
KW  -  proliferation
KW  -  apoptosis
KW  -  overexpression

DO  - 10.32604/biocell.2014.38.017