@Article{biocell.2015.39.005,
AUTHOR = {Rafael Martins de OLIVEIRA, Carolina Bernardi STEFANI, Angélica Aparecida Antoniellis SILVEIRA, Marcelo Lima RIBEIRO, José PEDRAZZOLI Jr., Alessandra GAMBERO},
TITLE = {Adenosine A1 receptor activation inhibits histamine release in gastric enterochromaffin-like cells},
JOURNAL = {BIOCELL},
VOLUME = {39},
YEAR = {2015},
NUMBER = {2-3},
PAGES = {5--8},
URL = {http://www.techscience.com/biocell/v39n2-3/34048},
ISSN = {1667-5746},
ABSTRACT = {Adenosine acts as a gastroprotective factor decreasing inflammation and reducing gastric
acid secretion. Quantitative RT-PCR was used to determine the expression of the adenosine receptor genes
<i>(A1AR, A2AAR, A2BAR and A3AR)</i> and that of the gastrin receptor B gene <i>(CCKBR)</i> in isolated, short-term
cultured enterochromaffin-like (ECL) cells. Both the A1AR and the <i>CCKBR</i> genes were expressed at a level higher than the other genes. Also, the effect of 2-chloroadenosine, a stable agonist of A1 and A2A receptors, was
explored on ECL cells, with a resulting inhibition of both basal and gastrin-stimulated histamine release. Also,
dipropylcyclopentylxanthine (DPCPX), a selective A1 antagonist, prevented the inhibitory effects of 2-chloroadenosine, suggesting the effect of 2-chloroadenosine is mediated by A1 receptors. It is concluded that isolated,
short-term cultured ECL cells are a suitable model for studies relating gene expression and function, and that the
gastroprotective actions of adenosine are at least partly mediated through A1 receptors.},
DOI = {10.32604/biocell.2015.39.005}
}