TY  - EJOU
AU  - OLIVEIRA, Rafael Martins de 
AU  - STEFANI, Carolina Bernardi 
AU  - SILVEIRA, Angélica Aparecida Antoniellis 
AU  - RIBEIRO, Marcelo Lima 
AU  - Jr., José PEDRAZZOLI 
AU  - GAMBERO, Alessandra 

TI  - Adenosine A1 receptor activation inhibits histamine release in gastric enterochromaffin-like cells
T2  - BIOCELL

PY  - 2015
VL  - 39
IS  - 2-3
SN  - 1667-5746

AB  - Adenosine acts as a gastroprotective factor decreasing inflammation and reducing gastric
acid secretion. Quantitative RT-PCR was used to determine the expression of the adenosine receptor genes
<i>(A1AR, A2AAR, A2BAR and A3AR)</i> and that of the gastrin receptor B gene <i>(CCKBR)</i> in isolated, short-term
cultured enterochromaffin-like (ECL) cells. Both the A1AR and the <i>CCKBR</i> genes were expressed at a level higher than the other genes. Also, the effect of 2-chloroadenosine, a stable agonist of A1 and A2A receptors, was
explored on ECL cells, with a resulting inhibition of both basal and gastrin-stimulated histamine release. Also,
dipropylcyclopentylxanthine (DPCPX), a selective A1 antagonist, prevented the inhibitory effects of 2-chloroadenosine, suggesting the effect of 2-chloroadenosine is mediated by A1 receptors. It is concluded that isolated,
short-term cultured ECL cells are a suitable model for studies relating gene expression and function, and that the
gastroprotective actions of adenosine are at least partly mediated through A1 receptors.
KW  - Gastrin
KW  -  2-chloroadenosine
KW  -  DPCPX

DO  - 10.32604/biocell.2015.39.005