@Article{biocell.2015.39.009,
AUTHOR = {Odin RAMÍREZ-FERNÁNDEZ, Rafael GODÍNEZ, Esmeralda ZUÑIGA-AGUILAR, Luis E. GÓMEZ-QUIROZ, María C. GUTIÉRREZ-RUIZ, Juan MORALES, Roberto OLAYO},
TITLE = {Hepatocyte culture in a radial-flow bioreactor with plasma polypyrrole coated scaffolds},
JOURNAL = {BIOCELL},
VOLUME = {39},
YEAR = {2015},
NUMBER = {2-3},
PAGES = {9--14},
URL = {http://www.techscience.com/biocell/v39n2-3/34049},
ISSN = {1667-5746},
ABSTRACT = {We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma
cell culture on a new porous coated scaffold. We designed a culture chamber where a radial flow of culture medium was continuously delivered through it. Once this system was established, flow was simulated using flow dynamics software based on numeric methods to solve Navier-Stockes flow equations. Perfusion within cell culture
scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed
unhindered in the bioreactor chamber. Afterwards, the bioreactor was built according to the simulated design
and was tested with liver carcinoma cells (HepG2) cultured over an L-polylactic acid scaffold whose surface was
modified with iodine-doped polypyrrole. The bioreactor was tested under non-flow and in radial flow conditions. Cell density under radial flow conditions was almost double than that under static conditions and both
total protein and albumin output was also increased under radial flow conditions. },
DOI = {10.32604/biocell.2015.39.009}
}