TY  - EJOU
AU  - BUSTAMANTE, Juanita 
AU  - CZERNICZYNIEC, Analía 
AU  - LORES-ARNAIZ, Silvia 

TI  - Ketamine effect on intracellular and mitochondrial calcium mobilization
T2  - BIOCELL

PY  - 2016
VL  - 40
IS  - 1
SN  - 1667-5746

AB  -  The suppressive effects of ketamine on intracellular calcium has been reported in a variety of cells
although the mechanisms involved are not well understood. The aim of this work was to evaluate the ketamine
effect on the mitochondrial Ca<sup>2+</sup> accumulation and the cellular Ca<sup>2+</sup> mobilization using FLUO4-AM and flow
cytometry. The results showed that mitochondria from ketamine injected animals presented a lower ability to
retain calcium at concentrations higher than 20 μM, as compared with controls (saline injected animals). In addition, ketamine showed a significant decreased KCl-induced intracellular calcium concentration. KCl increased
calcium influx through cellular depolarization. According to the data presented herein, ketamine presents a
clear inhibitory effect on cytosolic Ca<sup>2+</sup> transport mechanisms, independently from their action on the calcium
channel associated NMDA receptor.
KW  - calcium transport
KW  -  hippocampal mitochondria
KW  -  Vero cell culture

DO  - 10.32604/biocell.2016.40.011